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dox condition culture medium  (Thermo Fisher)


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    Thermo Fisher dox condition culture medium
    Selection of <t>dox-regulated</t> Tet-TNF-Cnx clones. Western blot analysis of Cnx in the selected overexpressing Tet-TNF-Cnx clones (2, 5, 6, 13, and 24) and a negative control cell (Tet-TNF-Null) to choose highly dox-regulatable clones. Cells were cultivated in the maintenance <t>medium</t> containing 1 μg/ml dox to regulate Cnx expression to a basal level. Two days after seeding, cells were cultivated for 24 h with 2 μg/ml dox or without dox after which they were sampled. Cell lysates were loaded at 5 × 104 cells/10 μl concentration. Relative band intensity was calculated using TINA 2.0 software. Regulation folds were calculated by dividing band intensity of 0 μg/ml dox <t>condition</t> by that of 2 μg/ml dox condition
    Dox Condition Culture Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dox condition culture medium/product/Thermo Fisher
    Average 97 stars, based on 475 article reviews
    dox condition culture medium - by Bioz Stars, 2026-03
    97/100 stars

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    1) Product Images from "Calnexin overexpression sensitizes recombinant CHO cells to apoptosis induced by sodium butyrate treatment"

    Article Title: Calnexin overexpression sensitizes recombinant CHO cells to apoptosis induced by sodium butyrate treatment

    Journal: Cell Stress & Chaperones

    doi: 10.1007/s12192-008-0054-0

    Selection of dox-regulated Tet-TNF-Cnx clones. Western blot analysis of Cnx in the selected overexpressing Tet-TNF-Cnx clones (2, 5, 6, 13, and 24) and a negative control cell (Tet-TNF-Null) to choose highly dox-regulatable clones. Cells were cultivated in the maintenance medium containing 1 μg/ml dox to regulate Cnx expression to a basal level. Two days after seeding, cells were cultivated for 24 h with 2 μg/ml dox or without dox after which they were sampled. Cell lysates were loaded at 5 × 104 cells/10 μl concentration. Relative band intensity was calculated using TINA 2.0 software. Regulation folds were calculated by dividing band intensity of 0 μg/ml dox condition by that of 2 μg/ml dox condition
    Figure Legend Snippet: Selection of dox-regulated Tet-TNF-Cnx clones. Western blot analysis of Cnx in the selected overexpressing Tet-TNF-Cnx clones (2, 5, 6, 13, and 24) and a negative control cell (Tet-TNF-Null) to choose highly dox-regulatable clones. Cells were cultivated in the maintenance medium containing 1 μg/ml dox to regulate Cnx expression to a basal level. Two days after seeding, cells were cultivated for 24 h with 2 μg/ml dox or without dox after which they were sampled. Cell lysates were loaded at 5 × 104 cells/10 μl concentration. Relative band intensity was calculated using TINA 2.0 software. Regulation folds were calculated by dividing band intensity of 0 μg/ml dox condition by that of 2 μg/ml dox condition

    Techniques Used: Selection, Clone Assay, Western Blot, Negative Control, Expressing, Concentration Assay, Software

    Culture of Tet-TNF-Cnx clones with regulated Cnx expression. A Culture profile of Tet-TNF-Null (Null cells). B Culture profile of Tet-TNF-Cnx 2 (Clone 2, dox-non-responsive clone). C Culture profile of Tet-TNF-Cnx 5 (Clone 5 with low basal level Cnx). D Culture profile of Tet-TNF-Cnx 6 (clone 6 with high basal level Cnx). Spent medium was replaced with fresh medium containing control without dox (filled triangle), control with dox (empty triangle), 5 mM NaBu without dox (filled circle), and 5 mM NaBu with dox (empty circle) on day 2. Culture supernatant was collected everyday and the cells on the designated days for Western blot analysis. Cell number and viability percent was determined using the trypan blue dye exclusion method. Protein titer was quantitated by ELISA. Arrows indicate the time of NaBu addition. The error bars represent the standard deviation calculated from the data obtained in duplicate experiments
    Figure Legend Snippet: Culture of Tet-TNF-Cnx clones with regulated Cnx expression. A Culture profile of Tet-TNF-Null (Null cells). B Culture profile of Tet-TNF-Cnx 2 (Clone 2, dox-non-responsive clone). C Culture profile of Tet-TNF-Cnx 5 (Clone 5 with low basal level Cnx). D Culture profile of Tet-TNF-Cnx 6 (clone 6 with high basal level Cnx). Spent medium was replaced with fresh medium containing control without dox (filled triangle), control with dox (empty triangle), 5 mM NaBu without dox (filled circle), and 5 mM NaBu with dox (empty circle) on day 2. Culture supernatant was collected everyday and the cells on the designated days for Western blot analysis. Cell number and viability percent was determined using the trypan blue dye exclusion method. Protein titer was quantitated by ELISA. Arrows indicate the time of NaBu addition. The error bars represent the standard deviation calculated from the data obtained in duplicate experiments

    Techniques Used: Clone Assay, Expressing, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Western blot analysis of Tet-TNF-Cnx clones for cellular proteins from batch culture with NaBu treatment. A Western blot data for Tet-TNF-Null (null cells). B Western blot data for Tet-TNF-Cnx 2 (clone 2, dox-non-responsive clone). C Western blot data for Tet-TNF-Cnx 5 (Clone 5 with low basal level Cnx). D Western blot data for Tet-TNF-Cnx 6 (Clone 6 with high basal level Cnx). Cells were loaded at a concentration of 1 × 105 cells/10 μl and resolved using the 4–20% Tris-glycine gel. Note the elevated cytochrome C release and PARP cleavage for clones 5 and 6 under Cnx-overexpressed condition (− dox)
    Figure Legend Snippet: Western blot analysis of Tet-TNF-Cnx clones for cellular proteins from batch culture with NaBu treatment. A Western blot data for Tet-TNF-Null (null cells). B Western blot data for Tet-TNF-Cnx 2 (clone 2, dox-non-responsive clone). C Western blot data for Tet-TNF-Cnx 5 (Clone 5 with low basal level Cnx). D Western blot data for Tet-TNF-Cnx 6 (Clone 6 with high basal level Cnx). Cells were loaded at a concentration of 1 × 105 cells/10 μl and resolved using the 4–20% Tris-glycine gel. Note the elevated cytochrome C release and PARP cleavage for clones 5 and 6 under Cnx-overexpressed condition (− dox)

    Techniques Used: Western Blot, Clone Assay, Concentration Assay



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    dox condition culture medium  (Thermo Fisher)
    97
    Thermo Fisher dox condition culture medium
    Selection of <t>dox-regulated</t> Tet-TNF-Cnx clones. Western blot analysis of Cnx in the selected overexpressing Tet-TNF-Cnx clones (2, 5, 6, 13, and 24) and a negative control cell (Tet-TNF-Null) to choose highly dox-regulatable clones. Cells were cultivated in the maintenance <t>medium</t> containing 1 μg/ml dox to regulate Cnx expression to a basal level. Two days after seeding, cells were cultivated for 24 h with 2 μg/ml dox or without dox after which they were sampled. Cell lysates were loaded at 5 × 104 cells/10 μl concentration. Relative band intensity was calculated using TINA 2.0 software. Regulation folds were calculated by dividing band intensity of 0 μg/ml dox <t>condition</t> by that of 2 μg/ml dox condition
    Dox Condition Culture Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dox condition culture medium/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    dox condition culture medium - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Selection of dox-regulated Tet-TNF-Cnx clones. Western blot analysis of Cnx in the selected overexpressing Tet-TNF-Cnx clones (2, 5, 6, 13, and 24) and a negative control cell (Tet-TNF-Null) to choose highly dox-regulatable clones. Cells were cultivated in the maintenance medium containing 1 μg/ml dox to regulate Cnx expression to a basal level. Two days after seeding, cells were cultivated for 24 h with 2 μg/ml dox or without dox after which they were sampled. Cell lysates were loaded at 5 × 104 cells/10 μl concentration. Relative band intensity was calculated using TINA 2.0 software. Regulation folds were calculated by dividing band intensity of 0 μg/ml dox condition by that of 2 μg/ml dox condition

    Journal: Cell Stress & Chaperones

    Article Title: Calnexin overexpression sensitizes recombinant CHO cells to apoptosis induced by sodium butyrate treatment

    doi: 10.1007/s12192-008-0054-0

    Figure Lengend Snippet: Selection of dox-regulated Tet-TNF-Cnx clones. Western blot analysis of Cnx in the selected overexpressing Tet-TNF-Cnx clones (2, 5, 6, 13, and 24) and a negative control cell (Tet-TNF-Null) to choose highly dox-regulatable clones. Cells were cultivated in the maintenance medium containing 1 μg/ml dox to regulate Cnx expression to a basal level. Two days after seeding, cells were cultivated for 24 h with 2 μg/ml dox or without dox after which they were sampled. Cell lysates were loaded at 5 × 104 cells/10 μl concentration. Relative band intensity was calculated using TINA 2.0 software. Regulation folds were calculated by dividing band intensity of 0 μg/ml dox condition by that of 2 μg/ml dox condition

    Article Snippet: Regulation folds were calculated by dividing band intensity of 0 μg/ml dox condition by that of 2 μg/ml dox condition Culture medium and maintenance The medium for culture maintenance of Tet-TNF-Cnx and Tet-TNF-Null cells was IMDM supplemented with 10% dialyzed fetal bovine serum (Gibco, Grand Island, NY, USA), 250 μg/ml hygromycin, 350 μg/ml zeocin, 1 μg/ml dox (Clontech), and 0.02 μM methotrexate (MTX, Sigma).

    Techniques: Selection, Clone Assay, Western Blot, Negative Control, Expressing, Concentration Assay, Software

    Culture of Tet-TNF-Cnx clones with regulated Cnx expression. A Culture profile of Tet-TNF-Null (Null cells). B Culture profile of Tet-TNF-Cnx 2 (Clone 2, dox-non-responsive clone). C Culture profile of Tet-TNF-Cnx 5 (Clone 5 with low basal level Cnx). D Culture profile of Tet-TNF-Cnx 6 (clone 6 with high basal level Cnx). Spent medium was replaced with fresh medium containing control without dox (filled triangle), control with dox (empty triangle), 5 mM NaBu without dox (filled circle), and 5 mM NaBu with dox (empty circle) on day 2. Culture supernatant was collected everyday and the cells on the designated days for Western blot analysis. Cell number and viability percent was determined using the trypan blue dye exclusion method. Protein titer was quantitated by ELISA. Arrows indicate the time of NaBu addition. The error bars represent the standard deviation calculated from the data obtained in duplicate experiments

    Journal: Cell Stress & Chaperones

    Article Title: Calnexin overexpression sensitizes recombinant CHO cells to apoptosis induced by sodium butyrate treatment

    doi: 10.1007/s12192-008-0054-0

    Figure Lengend Snippet: Culture of Tet-TNF-Cnx clones with regulated Cnx expression. A Culture profile of Tet-TNF-Null (Null cells). B Culture profile of Tet-TNF-Cnx 2 (Clone 2, dox-non-responsive clone). C Culture profile of Tet-TNF-Cnx 5 (Clone 5 with low basal level Cnx). D Culture profile of Tet-TNF-Cnx 6 (clone 6 with high basal level Cnx). Spent medium was replaced with fresh medium containing control without dox (filled triangle), control with dox (empty triangle), 5 mM NaBu without dox (filled circle), and 5 mM NaBu with dox (empty circle) on day 2. Culture supernatant was collected everyday and the cells on the designated days for Western blot analysis. Cell number and viability percent was determined using the trypan blue dye exclusion method. Protein titer was quantitated by ELISA. Arrows indicate the time of NaBu addition. The error bars represent the standard deviation calculated from the data obtained in duplicate experiments

    Article Snippet: Regulation folds were calculated by dividing band intensity of 0 μg/ml dox condition by that of 2 μg/ml dox condition Culture medium and maintenance The medium for culture maintenance of Tet-TNF-Cnx and Tet-TNF-Null cells was IMDM supplemented with 10% dialyzed fetal bovine serum (Gibco, Grand Island, NY, USA), 250 μg/ml hygromycin, 350 μg/ml zeocin, 1 μg/ml dox (Clontech), and 0.02 μM methotrexate (MTX, Sigma).

    Techniques: Clone Assay, Expressing, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Western blot analysis of Tet-TNF-Cnx clones for cellular proteins from batch culture with NaBu treatment. A Western blot data for Tet-TNF-Null (null cells). B Western blot data for Tet-TNF-Cnx 2 (clone 2, dox-non-responsive clone). C Western blot data for Tet-TNF-Cnx 5 (Clone 5 with low basal level Cnx). D Western blot data for Tet-TNF-Cnx 6 (Clone 6 with high basal level Cnx). Cells were loaded at a concentration of 1 × 105 cells/10 μl and resolved using the 4–20% Tris-glycine gel. Note the elevated cytochrome C release and PARP cleavage for clones 5 and 6 under Cnx-overexpressed condition (− dox)

    Journal: Cell Stress & Chaperones

    Article Title: Calnexin overexpression sensitizes recombinant CHO cells to apoptosis induced by sodium butyrate treatment

    doi: 10.1007/s12192-008-0054-0

    Figure Lengend Snippet: Western blot analysis of Tet-TNF-Cnx clones for cellular proteins from batch culture with NaBu treatment. A Western blot data for Tet-TNF-Null (null cells). B Western blot data for Tet-TNF-Cnx 2 (clone 2, dox-non-responsive clone). C Western blot data for Tet-TNF-Cnx 5 (Clone 5 with low basal level Cnx). D Western blot data for Tet-TNF-Cnx 6 (Clone 6 with high basal level Cnx). Cells were loaded at a concentration of 1 × 105 cells/10 μl and resolved using the 4–20% Tris-glycine gel. Note the elevated cytochrome C release and PARP cleavage for clones 5 and 6 under Cnx-overexpressed condition (− dox)

    Article Snippet: Regulation folds were calculated by dividing band intensity of 0 μg/ml dox condition by that of 2 μg/ml dox condition Culture medium and maintenance The medium for culture maintenance of Tet-TNF-Cnx and Tet-TNF-Null cells was IMDM supplemented with 10% dialyzed fetal bovine serum (Gibco, Grand Island, NY, USA), 250 μg/ml hygromycin, 350 μg/ml zeocin, 1 μg/ml dox (Clontech), and 0.02 μM methotrexate (MTX, Sigma).

    Techniques: Western Blot, Clone Assay, Concentration Assay